Key factors of centrifuge centrifugation technology

The design principle of the centrifuge is to use the centrifugal field force generated when the rotating head rotates to accelerate the sedimentation speed of the sample particles and separate substances with different sedimentation coefficients or buoyant densities in the sample.In addition to rotations per minute (r/min) and centrifugal (rotation) radius, the factors that determine the size of the centrifugal force are also related to the influence of the forces of the particles (gravity, buoyancy, friction).Rotational motion, centrifugal force.The direction is perpendicular to gravity, so the relative centrifugal force (R0) is used, which is the centrifugal force acting on the rotating particle relative to gravity, and the gravitational acceleration g (980cm/s) is used as the amplitude, also known as 'g-Force'.', the expression is: RCF=o)~/980.


　　The key to centrifugation is how to determine the centrifugal force, speed and centrifugation time settings based on the properties of the sample particles and media and the technical parameters of the rotor.The rate of sedimentation or separation of particles (collectively referred to as cells, organelles, and macromolecules) depends on centrifugal force, particle size, shape, and density, and the density and viscosity of the settling medium.Several commonly used centrifuges are available depending on the application, speed and functional configuration.: Medical centrifuge, laboratory centrifuge, cosmetic centrifuge, PRP centrifuge, high-speed centrifuge, low-speed centrifuge, high-speed refrigerated centrifuge, low-speed refrigerated centrifuge, etc. Centrifuges with different speeds produce different centrifugal forces, different centrifugal forces Used for.


　　Choosing the right centrifuge can not only solve production problems and improve work efficiency, but also save production costs and reduce operational risks.The main factors affecting choice are as follows:


　　1. Centrifuge speed: Generally, the centrifuge speed should be higher than 3000 rpm.The higher the speed, the higher the separation coefficient of the centrifuge and the better the separation effect.


　　2. Centrifuge materials: Different materials have different physical and chemical indicators, such as wear resistance and corrosion resistance.Exotic centrifuges typically have low materials of 316L or duplex stainless steel, and the abrasive component must be made of ceramic composite.


　　3. Differential control of centrifuge: Different differentials have different control accuracy, and the difference between lifespan and maintenance cost is very large.The higher the differential accuracy, the better the adaptability to materials.Therefore, it is best to use a device with high differential accuracy..


　　4. Aspect ratio: The larger the aspect ratio of the centrifuge, the greater the processing capacity and the lower the moisture content.


　　5. Control system: Whether it is automatic programming control, at present, domestic and foreign equipment manufacturers have basically realized fully automated control of equipment.


　　6. Installation power: Energy consumption control is affected, equipment energy consumption ratio is high, and foreign energy consumption ratio is low.


　　7. Processing and production processes: Centrifuges are separation equipment required for high-precision processing.The products produced by the enterprise have no finishing capabilities, high maintenance rates, and limited processing capabilities.


　　An important indicator for measuring the separation performance of a centrifuge is the separation factor.It represents the ratio of centrifugal force to the gravity of the separated material in the drum.The larger the separation factor, the faster the separation is usually and the better the separation effect.The separation coefficient of industrial centrifuges is generally 100~20000, the separation coefficient of ultra-high-speed tubular separators can be as high as 62000, and the separation coefficient of analytical ultra-speed separators is as high as 610000. Another factor that determines the processing capacity of the centrifuge is the working area of ​​the drum , which also has large processing capabilities.