The design principle of the centrifuge is to use the centrifugal field force generated when the rotating head rotates to accelerate the sedimentation speed of the sample particles and separate substances with different sedimentation coefficients or buoyant densities in the sample.In addition to rotations per minute (r/min) and centrifugal (rotation) radius, the factors that determine the size of the centrifugal force are also related to the influence of the forces of the particles (gravity, buoyancy, friction).Rotational motion, centrifugal force.The direction is perpendicular to gravity, so the relative centrifugal force (R0) is used, which is the centrifugal force acting on the rotating particle relative to gravity, and the gravitational acceleration g (980cm/s) is used as the amplitude, also known as 'g-Force'.', the expression is: RCF=o)~/980. The key to centrifugation is how to determine the centrifugal force, speed and centrifugation time settings based on the properties of the sample particles and media and the technical parameters of the rotor. The sedimentation or separation rate of the particles (collectively known as cells, organelles and macromolecules) depend on the